In vitro Antioxidant Activity of Porana paniculata and Ipomoea quamoclit-Two Ethnomedicinally Important Plants of Convolvulaceae Family

Aims: Present study deals with the in vitro antioxidant activity of Porana paniculata and Ipomoea quamoclit whole plants belongs to Convolvulaceae family.
Study Design: The present study was designed for in vitro evaluation of antioxidant activity of two ethnomedicinally important plants of Convolvulaceae family.
Place and Duration of Study: Division of Pharmacognosy, Raghavendra Institute of Pharmaceutical Education and Research, Krishnam Reddy Palli cross, Chiyyedu, Anantapuramu-515721, Andhra Pradesh, India, between June 2013 and July 2013.
Methodology: Plant materials were procured, shade dried and made into powder. The extraction was carried out by maceration using water and ethanol as solvent and then total flavonoid and total phenol content was determined by standard methods. In vitro antioxidant activity was performed by DPPH assay, superoxide anion scavenging activity assay, nitric oxide scavenging activity assay, hydrogen peroxide scavenging assay and metal chelating activity. Percentage inhibition of free radical formation and IC50 values were calculated.
Results: Total flavonoids were found to be 59.86 and 49.26 for HAPP and HAIQ mg/ml of standard compound quercetin and total phenols was found to be 33.34 and 39.32 mg/g of gallic acid. In DPPH method, the half inhibition concentration [IC50] of HAPP, HAIQ and ascorbic acid were 71.68, 49.63 and 32.46 μg/ml respectively. In Superoxide anion scavenging activity assay, the half inhibition concentration [IC50] of HAPP, HAIQ and standard compound BHA was found to be 54.61, 69.34 and 93.46 μg/ml respectively. In nitric oxide scavenging activity assay both the extracts HAPP and HAIQ moderately inhibited nitric oxide in dose dependent manner with the IC50 being 83.49 and 89.47 μg/ml and standard compound ascorbic acid was 78.93 μg/ml respectively. The IC50 of HAPP, HAIQ and gallic acid were found 25.65, 46.39 and 24.29 μg/ml respectively in hydrogen peroxide scavenging method and in metal chelating method, HAPP and HAIQ indicated that they have chelating activity with an IC50 of 49.27 and 65.41 μg/ml and alpha tocopherol was 32.48 μg/ml.
Conclusion: From the above findings it can be concluded that Porana paniculata and Ipomoea quamoclit extracts exhibited antioxidant activity.