Study on the Extraction and Antitumor Activity of Achyranthes Bidentata Polysaccharide

Achyranthes bidentata polysaccharides (ABPS), a major active ingredient of Achyranthes bidentata, has many kinds of well-known pharmacological activity, such as anti-coagulation, indirect anti-tumor, anti-aging and hypoglycemic effect, and also have certain effects on the immune system. However, the direct anti-tumor activity of ABPS remain unclear. In this paper, ABPS was efficiently isolated from Achyranthes bidentata adopted orthogonal experimental method, and then its anti-tumor effect on LTEP-a-2 cells was investigated. LTEP-a-2 cells were treated with various concentrations of ABPS (0, 0.2, 0.4, 0.6, 0.8 and 1 mg/mL) for 48h. Subsequently, cell viability was analyzed by MTT method and the effects of ABPS on the apoptosis of LTEP-a-2 cells was explored by DNA ladder, PI and Annexin V FITC/PI double staining. The results showed that the optimum extraction conditions of ABPS was that the extraction temperature at 90oC, 1:40(W/V) of solid to liquid, and extraction time 4 h. In addition, the yield and purity of ABP was measured up to 15.52% and 97.21% respectively. Furthermore, ABPS significantly inhibited the proliferation and induced the apoptosis of LTEP-a-2 cells. So ABPS might be utilized as a new potential therapeutic agent against Lung Adenocarcinoma.